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验证数据展示

  • WB analysis using 87237-1-RR

    WB analysis using 87237-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87237-1-RR (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 87237-1-RR

    WB analysis of A431 using 87237-1-RR

    WB result of RAB11A/B antibody (87237-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A/B transfected A431 cells.

  • IP experiment of mouse spleen using 87237-1-RR

    IP experiment of mouse spleen using 87237-1-RR

    IP result of anti-RAB11A/B (IP:87237-1-RR, 4ug; Detection:87237-1-RR 1:3000) with mouse spleen tissue lysate 2000 ug.

  • KD/KO Validated

RAB11A/B Recombinant monoclonal antibody

RAB11A/B Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 87237-1-RR

产品说明书

CloneNo. 252492E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Rab 11, RAB11, RAB11A, EC:3.6.5.2, Rab-11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy RAB11A/B Ready-To-Use IHC Kit. RAB11A/B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87237-1-RR targets RAB11A/B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8742

Product name: Recombinant human RAB11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC013348

Sequence: MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB11A, member RAS oncogene family
别名 Rab 11, RAB11, RAB11A, EC:3.6.5.2, Rab-11
计算分子量 216 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC013348
基因名称 RAB11A
Gene ID (NCBI) 8766
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62491
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB11 has two isoform genes RAB11A(YL8) and RAB11B(YPT3), and belongs to the small GTPase superfamily and Rab family. It regulates endocytic recycling. RAB11 may exert its functions by interacting with multiple effector proteins in different complexes. It acts as a major regulator of membrane delivery during cytokinesis. RAB11 regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. This antibody can recognize both RAB11A(24 kDa) and RAB11B(24 kDa).

实验方案

Product Specific Protocols
IP protocol for RAB11A/B antibody 87237-1-RRDownload protocol
WB protocol for RAB11A/B antibody 87237-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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