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验证数据展示

  • WB analysis using 86787-1-RR (same clone as 86787-1-PBS)

    WB analysis using 86787-1-RR (same clone as 86787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86787-1-RR (RAB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86787-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 86787-1-RR (same clone as 86787-1-PBS)

    IF Staining of MCF-7 using 86787-1-RR (same clone as 86787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using RAB10 antibody (86787-1-RR, Clone: 251744G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86787-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86787-1-RR

    Affinity and Kinetic Characterization of 86787-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86787-1-RR against Human RAB10 were performed. The affinity constant is 3.91 nM.

RAB10 Recombinant monoclonal antibody, PBS Only

RAB10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86787-1-PBS

产品说明书

CloneNo. 251744G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAB10 Ready-To-Use IHC Kit. RAB10 primary antibody included.

产品信息

86787-1-PBS targets RAB10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25830

Product name: Recombinant human RAB10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-200 aa of BC000896

Sequence: RVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB10, member RAS oncogene family
别名 EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10
计算分子量 200 aa, 23 kDa
观测分子量20-23 kDa
GenBank蛋白编号BC000896
基因名称 RAB10
Gene ID (NCBI) 10890
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61026
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.

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