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验证数据展示

  • WB analysis using 86333-3-RR (same clone as 86333-3-PBS)

    WB analysis using 86333-3-RR (same clone as 86333-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86333-3-RR (QARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86333-3-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 86333-3-RR (same clone as 86333-3-PBS)

    IF Staining of NIH/3T3 using 86333-3-RR (same clone as 86333-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using QARS antibody (86333-3-RR, Clone: 251052A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86333-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86333-3-RR

    Affinity and Kinetic Characterization of 86333-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86333-3-RR against Human QARS were performed. The affinity constant is 0.176 nM.

QARS Recombinant monoclonal antibody, PBS Only

QARS Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86333-3-PBS

产品说明书

CloneNo. 251052A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

GlnRS, EC:6.1.1.18, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, QARS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® GlnRS Ready-To-Use IHC Kit. GlnRS primary antibody included.

产品信息

86333-3-PBS targets QARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3375

Product name: Recombinant human QARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 362-717 aa of BC016634

Sequence: VKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutaminyl-tRNA synthetase
别名 GlnRS, EC:6.1.1.18, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, QARS1
计算分子量 775 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC016634
基因名称 GlnRS
Gene ID (NCBI) 5859
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP47897
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

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