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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

    WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Prohibitin/PHB1 antibody included
  • IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Prohibitin/PHB1 antibody included

Prohibitin Monoclonal antibody, PBS Only

Prohibitin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60092-1-PBS

产品说明书

CloneNo. 4D3G5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, Indirect ELISA

别名

PHB, 4D3G5, PHB1, Prohibitin 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Prohibitin/PHB1 Ready-To-Use IHC Kit. Prohibitin/PHB1 primary antibody included.

产品信息

60092-1-PBS targets Prohibitin in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag1240

Product name: Recombinant human Prohibitin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC013401

Sequence: MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prohibitin
别名 PHB, 4D3G5, PHB1, Prohibitin 1
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC013401
基因名称 Prohibitin
Gene ID (NCBI) 5245
RRIDAB_2252403
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35232
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).