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验证数据展示

  • WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (PENK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Fetal human brain was subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 32272-1-AP (same clone as 32272-1-PBS)

    IF Staining of SH-SY5Y using 32272-1-AP (same clone as 32272-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Proenkephalin-A antibody (32272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

Proenkephalin-A Polyclonal antibody, PBS Only

Proenkephalin-A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32272-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PENK, Proenkephalin A, Opioid growth factor, Met-enkephalin-Arg-Phe, Met-enkephalin-Arg-Gly-Leu

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32272-1-PBS targets Proenkephalin-A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27972

Product name: Recombinant human PENK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-267 aa of BC032505

Sequence: YGGFMKRYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKELLETGDNRERSHHQDGSDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFAEALPSDEEGESYSKEVPEMEKRYGGFMRF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proenkephalin
别名 PENK, Proenkephalin A, Opioid growth factor, Met-enkephalin-Arg-Phe, Met-enkephalin-Arg-Gly-Leu
计算分子量 267 aa, 31 kDa
观测分子量 32 kDa
GenBank蛋白编号BC032505
基因名称 Proenkephalin A
Gene ID (NCBI) 5179
RRIDAB_3742560
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP01210
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035). Proenkephalin (PENK) represents a new candidate to determine kidney function. This peptide is cleaved from the precursor peptide preproenkephalin A alongside enkephalins (endogenous opioids) and is filtrated in the glomerulus (PMID: 33636859).

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