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Podocalyxin Monoclonal antibody, PBS Only

Podocalyxin Monoclonal Antibody for Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

4A3C7

Cat No : 68250-1-PBS

Print datasheet

Synonyms

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

验证数据展示

  • IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Podocalyxin antibody included
  • IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Podocalyxin antibody included
  • IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68250-1-Ig (same clone as 68250-1-PBS)

    FC experiment of HeLa using 68250-1-Ig (same clone as 68250-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Podocalyxin (68250-1-Ig, Clone:4A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Podocalyxin Ready-To-Use IHC Kit. Podocalyxin primary antibody included.

产品信息

68250-1-PBS targets Podocalyxin in IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications Indirect ELISA,IHC,IF,FC
Tested Reactivity Human
Immunogen Podocalyxin fusion protein Ag12859 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name podocalyxin-like
Synonyms GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60
Calculated Molecular Weight 526 aa, 55 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC093730
Gene Symbol PODXL
Gene ID (NCBI) 5420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00592
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).