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验证数据展示

  • WB analysis of A549 using 10602-1-AP (same clone as 10602-1-PBS)

    WB analysis of A549 using 10602-1-AP (same clone as 10602-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

    IP experiment of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

    IP result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

    IF Staining of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Plexin-B2 Polyclonal antibody, PBS Only

Plexin-B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10602-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Plexin B2, PLXNB2, KIAA0315, MM1, Nbla00445

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10602-1-PBS targets Plexin-B2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0941

Product name: Recombinant human PLXNB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1542-1841 aa of BC004542

Sequence: WKRVNTLMHYNVRDGATLILSKVGVSQQPEDSQQDLPGERHALLEEENRVWHLVRPTDEVDEGKSKRGSVKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLAPGHAVPPAVKYFFDFLDEQAEKHNIQDEDTIHIWKTNSLPLRFWVNILKNPHFIFDVHVHEVVDASLSVIAQTFMDACTRTEHKLSRDSPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTDSLNTLVALHQLYQYTQKYYDEIINALEEDPAAQKMQLAFRLQQIAAALENKVTDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plexin B2
别名 Plexin B2, PLXNB2, KIAA0315, MM1, Nbla00445
计算分子量 205 kDa
观测分子量 80 kDa, 240 kDa
GenBank蛋白编号BC004542
基因名称 PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2166424
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15031
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

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