Phospho-mTOR (Ser2448) Monoclonal antibody
Phospho-mTOR (Ser2448) Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat and More (6)
Applications
IF, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
174
CloneNo.
2A12G3
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, HEK-293T cells, HEK-293 cells, HSC-T6 cells, Calyculin A treated HeLa cells, EGF treated NIH/3T3 cells, Rapamycin treated HEK-293 cells, Calyculin A treated HEK-293 cells |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67778-1-Ig targets Phospho-mTOR (Ser2448) in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF, IHC, WB, ELISA |
Cited Applications | Cell treatment, IF, IHC, WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat, chicken, pig, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1 |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 289 kDa |
GenBank Accession Number | BC117166 |
Gene Symbol | FRAP1 |
Gene ID (NCBI) | 2475 |
RRID | AB_2889842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig | Download protocol |
IHC protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig | Download protocol |
IF protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Redox Biol GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux | ||
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization | ||
J Control Release cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer | ||
Mater Today Bio Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy. | ||
Proc Natl Acad Sci U S A Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy |