验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30811-1-AP targets Phospho-eEF2 (Thr57) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic elongation factor-2 kinase |
| 别名 | eEF2 (Thr57), EEF2(Thr57), p eEF2, p eEF2 (Thr57), p eEF2 Thr57 |
| 计算分子量 | 725 aa, 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC032665 |
| 基因名称 | EEF2K |
| Gene ID (NCBI) | 29904 |
| RRID | AB_3669761 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00418 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-eEF2 (Thr57) antibody 30811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
