验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy YBX1 Ready-To-Use IHC Kit. YBX1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84993-5-RR targets Phospho-YBX1 (Ser102) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Y box binding protein 1 |
| 别名 | p YB1 (Ser102), Phospho YB1 (Ser102), Phospho YBX1, Phospho YBX1 (Ser102), Phospho-YB1 |
| 计算分子量 | 324 aa, 36 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC010430 |
| 基因名称 | YBX1 |
| Gene ID (NCBI) | 4904 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P67809 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YBX1, also known as Y-box binding protein 1, is a multifunctional DNA- and RNA-binding protein and transcription factor that plays a crucial role in various cellular processes, including gene expression, RNA metabolism, and cellular stress response. The phosphorylation of YBX1 at serine 102 (Ser102) is a key post-translational modification that significantly impacts its function and localization within the cell. (PMID: 34680946)
