Phospho-YAP1 (Ser397) Polyclonal antibody
Phospho-YAP1 (Ser397) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Serum-free treated NIH/3T3 cells, 10 % fetal bovine serum treated NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
29018-1-AP targets Phospho-YAP1 (Ser397) in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Yes-associated protein 1, 65kDa |
| Synonyms | 65 kDa Yes associated protein, Phospho-YAP1 (Ser397), YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |
| Calculated Molecular Weight | 504 aa, 54 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC038235 |
| Gene Symbol | YAP1 |
| Gene ID (NCBI) | 10413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46937 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-YAP1 (Ser397) antibody 29018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective | ||
Arch Biochem Biophys Oridonin suppresses the growth of glioblastoma cells via inhibiting Hippo/YAP axis | ||
J Nat Med Ruscogenin ameliorates dasatinib-induced intestinal barrier dysfunction via ErbB4/YAP and ROCK/MLC pathways | ||
Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling | ||
Mol Carcinog LATS-regulated nuclear-cytoplasmic translocation of SREBP2 inhibits hepatocellular carcinoma cell migration and invasion via epithelial-mesenchymal transition | ||
Biochem Biophys Res Commun IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression |