验证数据展示
经过测试的应用
| Positive WB detected in | Forskolin treated NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy® VASP Ready-To-Use IHC Kit. VASP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87168-2-RR targets Phospho-VASP (Ser157) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | vasodilator-stimulated phosphoprotein |
| 别名 | Phospho VASP (Ser157), Phospho-VASP, Phospho-VASP-Ser157, p-VASP (S157), p-VASP (Ser157) |
| 计算分子量 | 380 aa, 40 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC038224 |
| 基因名称 | VASP |
| Gene ID (NCBI) | 7408 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P50552 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VASP belongs to the Ena/VASP family. Phospho-VASP (Ser157) is an important post-translational modification of the vasodilator-stimulated phosphoprotein (VASP). Phosphorylation at Serine 157 (Ser157) is catalyzed by protein kinase C (PKC) and Rho kinase in response to thrombin or phorbol ester stimulation in human platelets. This modification negatively regulates platelet adhesion and secretion, and serves as a key signaling event in modulating cytoskeletal dynamics and cell motility. (PMID: 16197368)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-VASP (Ser157) antibody 87168-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
