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验证数据展示

  • IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis using 80007-1-RR (same clone as 80007-1-PBS)

    WB analysis using 80007-1-RR (same clone as 80007-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80007-1-RR (same clone as 80007-1-PBS)

    IF Staining of HeLa using 80007-1-RR (same clone as 80007-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-TDP43 (Ser409/410) antibody (80007-1-RR, Clone: 6M10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 81350-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

  • Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

Phospho-TDP43 (Ser409/410) Recombinant monoclonal antibody, PBS Only (Capture)

Phospho-TDP43 (Ser409/410) Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 80007-1-PBS

产品说明书

CloneNo. 6M10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

p TDP43, p TDP43 (Ser409/410), p TDP43 Ser409/410, Phospho TDP43, Phospho TDP43 (Ser409/410)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

产品信息

80007-1-PBS targets Phospho-TDP43 (Ser409/410) as part of a matched antibody pair:

MP00589-1: 80007-1-PBS capture and 81350-1-PBS detection (validated in Cytometric bead array)

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TAR DNA binding protein
别名 p TDP43, p TDP43 (Ser409/410), p TDP43 Ser409/410, Phospho TDP43, Phospho TDP43 (Ser409/410)
计算分子量 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_007375
基因名称 TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882937
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13148
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

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