验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
82383-1-RR targets Phospho-TBK1 (Ser172) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | TANK-binding kinase 1 |
| 别名 | FLJ11330, NAK, NF kappa B activating kinase, Phospho-TBK1 (Ser172), T2K, TANK binding kinase 1, TBK1 |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC034950 |
| 基因名称 | TBK1 |
| Gene ID (NCBI) | 29110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UHD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TBK1 (Ser172) antibody 82383-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation | ||
Eur J Pharmacol Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway | ||
Cell Rep Non-canonical isoforms of the mRNA polyadenylation factor WDR33 regulate STING-mediated immune responses | ||
Br J Pharmacol Glycyrrhetinic acid blocks SARS-CoV-2 infection by activating the cGAS-STING signalling pathway | ||
J Virol Pseudorabies virus infection triggers mitophagy to dampen the interferon response and promote viral replication | ||
Int J Biol Macromol Inhibiting UGCG prevents PRV infection by decreasing lysosome-associated autophage |