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验证数据展示

  • IHC staining of human brain（AD） using 87428-1-RR

    IHC staining of human brain（AD） using 87428-1-RR

    Immunohistochemical analysis of paraffin-embedded Normal human brain tissue (left) and human Alzheimer's disease brain(AD) slide (right) using 87428-1-RR (Phospho-TAU (Thr217) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 87428-1-RR

    WB analysis using 87428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of / using 87428-1-RR

    Dot Blot experiment of / using 87428-1-RR

    Dot blot analysis was used to confirm the specificity of 87428-1-RR,Phospho-TAU (Thr217) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (25ng) spotted is indicated next to each row.

Phospho-TAU (Thr217) Recombinant monoclonal antibody

Phospho-TAU (Thr217) Uni-rAb® Recombinant Antibody for WB, IHC, Dot Blot, ELISA
Cat No. 87428-1-RR

产品说明书

CloneNo. 252442E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Dot Blot, ELISA

别名

p Tau, p Tau (Thr217), p Tau Thr217, phos TAU, Phospho Tau

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse blood
Positive IHC detected inhuman brain (AD), human large intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive Dot Blot detected in/ /

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87428-1-RR targets Phospho-TAU (Thr217) in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 p Tau, p Tau (Thr217), p Tau Thr217, phos TAU, Phospho Tau
计算分子量 37-46, 79-81 kDa
观测分子量60-80 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau plays a crucial role in promoting and stabilizing microtubule assembly within neuronal axons. Phospho-TAU(Thr217) has emerged as a highly specific early biomarker for Alzheimer's disease (AD), detectable in both CSF and blood, effectively distinguishing AD from other dementias. This site is primarily phosphorylated by CDK5 and GSK-3β. Beyond its diagnostic value, p-T217 hyperphosphorylation is directly linked to synaptic dysfunction, neuronal loss, and cognitive decline in AD pathogenesis.

实验方案

Product Specific Protocols
IHC protocol for Phospho-TAU (Thr217) antibody 87428-1-RRDownload protocol
WB protocol for Phospho-TAU (Thr217) antibody 87428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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