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验证数据展示

  • WB analysis using 31138-1-AP

    WB analysis using 31138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

  • WB analysis using 31138-1-AP

    WB analysis using 31138-1-AP

    Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-TAU (Thr217) Polyclonal antibody

Phospho-TAU (Thr217) Polyclonal Antibody for WB, ELISA
Cat No. 31138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

p Tau, p Tau (Thr217), p Tau Thr217, Phospho Tau, Phospho Tau (Thr217)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inFAD3T mouse brain tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31138-1-AP targets Phospho-TAU (Thr217) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein tau
别名 p Tau, p Tau (Thr217), p Tau Thr217, Phospho Tau, Phospho Tau (Thr217)
计算分子量 37-46, 79-81 kDa
观测分子量70 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Phosphorylated tau at threonine 217 (p-tau217) is one of the emerging tau markers that has repeatedly shown more promise than other p-tau isoforms as a marker of AD pathology.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAU (Thr217) antibody 31138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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