验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87019-1-RR targets Phospho-TAU (Ser409) in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein tau |
| 别名 | p TAU, p TAU (Ser409), p TAU Ser409, phos TAU, Phospho TAU |
| 计算分子量 | 37-46, 79-81 kDa |
| 观测分子量 | 45-60 kDa |
| GenBank蛋白编号 | BC000558 |
| 基因名称 | TAU |
| Gene ID (NCBI) | 4137 |
| RRID | AB_3745278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10636 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphorylation of tau at Ser409 is an early, disease-specific event in Alzheimer's brain. Catalyzed by PKA, this modification weakens tau-microtubule binding and acts as a molecular switch that initiates tau self-aggregation into paired helical filaments. Consequently, p-Ser409-tau is recognized as an early diagnostic epitope and a potential target for interrupting PKA-driven, oxidative-stress-linked tau pathology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-TAU (Ser409) antibody 87019-1-RR | Download protocol |
| WB protocol for Phospho-TAU (Ser409) antibody 87019-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
