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验证数据展示

  • WB analysis using 86995-1-RR

    WB analysis using 86995-1-RR

    Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 86995-1-RR (Phospho-TAU (Ser199) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of mouse brain using 86995-1-RR

    IF Staining of mouse brain using 86995-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Ser199) antibody (86995-1-RR, Clone: 252075E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospho-TAU (Ser199) Recombinant monoclonal antibody

Phospho-TAU (Ser199) Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 86995-1-RR

产品说明书

CloneNo. 252075E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

p TAU, p TAU (Ser199), p TAU Ser199, phos TAU, Phospho TAU

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86995-1-RR targets Phospho-TAU (Ser199) in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 p TAU, p TAU (Ser199), p TAU Ser199, phos TAU, Phospho TAU
计算分子量 37-46, 79-81 kDa
观测分子量35-60 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_3745263
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Phospho-TAU (Ser199) antibody 86995-1-RRDownload protocol
WB protocol for Phospho-TAU (Ser199) antibody 86995-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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