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Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7G5

Cat No : 68000-1-Ig

Print datasheet

Synonyms

MGF, Phospho-STAT5A (Tyr694), STAT5, STAT5A

验证数据展示

  • WB analysis using 68000-1-Ig

    WB analysis using 68000-1-Ig

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 68000-1-lg (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

经过测试的应用

Positive WB detected inTF-1 cells, GM-CSF treated TF-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT5A Ready-To-Use IHC Kit. STAT5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68000-1-Ig targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 5A
Synonyms MGF, Phospho-STAT5A (Tyr694), STAT5, STAT5A
Calculated Molecular Weight 794 aa, 92 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2918748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody 68000-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Rep

Copper (II) complex of salicylate phenanthroline induces the apoptosis of colorectal cancer cells, including oxaliplatin‑resistant cells

Authors - Zixin Liu
View Article