Phospho-STAT3 (Ser727) Polyclonal antibody

Phospho-STAT3 (Ser727) Polyclonal Antibody for WB, ELISA
Cat No. 28945-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, ELISA and More (1)

p-STAT3, Phospho-STAT3, p STAT3 (Ser727), p-STAT3 (Ser727), STAT3 (Ser727)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28945-1-AP targets Phospho-STAT3 (Ser727) in WB, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse, rat, bovine
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducer and activator of transcription 3 (acute-phase response factor)
别名 p-STAT3, Phospho-STAT3, p STAT3 (Ser727), p-STAT3 (Ser727), STAT3 (Ser727)
计算分子量 770 aa, 88 kDa
观测分子量92-100 kDa
GenBank蛋白编号BC000627
基因名称 STAT3
Gene ID (NCBI) 6774
RRIDAB_3206262
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP40763
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization

Authors - Zhou Ji
mouseWB

Bioorg Chem

N-Heterocyclic functionalized chalcone derivatives as anti-inflammatory agents for atopic dermatitis treatment by inhibiting JAK1/STAT3 signaling pathway

Authors - Zhengxiao Huang
humanWB

World J Surg Oncol

Targeting NETO2 suppresses cell proliferation, invasion, and migration and inactivates the STAT3/C-MYC pathway in hepatocellular carcinoma

Authors - Na Shun Meng He
humanWB

Biochem Pharmacol

N6-methyladenosine-modified RNF220 induces cisplatin resistance and immune escape via regulating PDE10A K48-linked ubiquitination in bladder cancer

Authors - Kai Li
mouseWB

Genes Dis

Inhibition of sphingosine 1-phosphate receptor 3 ameliorates bleomycin-induced pulmonary fibrosis by suppressing macrophage M2 polarization

Authors - Huijun Qiu
mouse,bovineWB,IF

Redox Biol

Quercetin alleviates LPS/iE-DAP-induced liver injury by suppressing ferroptosis via regulating ferritinophagy and intracellular iron efflux

Authors - Hongzhu Zhang
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