验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, λ phosphatase treated HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28945-1-AP targets Phospho-STAT3 (Ser727) in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 3 (acute-phase response factor) |
| 别名 | p STAT3, p STAT3 (Ser727), p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727) |
| 计算分子量 | 770 aa, 88 kDa |
| 观测分子量 | 92-100 kDa |
| GenBank蛋白编号 | BC000627 |
| 基因名称 | STAT3 |
| Gene ID (NCBI) | 6774 |
| RRID | AB_3206262 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40763 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization | ||
Bioorg Chem N-Heterocyclic functionalized chalcone derivatives as anti-inflammatory agents for atopic dermatitis treatment by inhibiting JAK1/STAT3 signaling pathway | ||
Neuropharmacology Pantothenic acid-mediated inhibition of microglial inflammation via the JAK2/STAT3 pathway enhances motor function recovery after Spinal cord injury | ||
Int Immunopharmacol Dental pulp stem cells promote the recovery of spinal cord injury by regulating microglia polarization via JAK2/STAT3 signaling pathway | ||
Biochim Biophys Acta Mol Cell Res COA6 deficiency inhibits hepatocellular carcinoma progression by regulating cuproptosis through the JAK/STAT signaling pathway | ||
Immunobiology Phellodendrine chloride alleviates gouty arthritis through IL-6/STAT3 signaling pathway |
