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验证数据展示

  • WB analysis using 81219-2-RR

    WB analysis using 81219-2-RR

    Non-treated C6 cells and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 81219-2-RR

    WB analysis using 81219-2-RR

    Non-treated NIH/3T3 cells and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody

Phospho-SHP2 (Tyr580) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 81219-2-RR

产品说明书

CloneNo. 242434E2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPDGF treated NIH/3T3 cells, PDGF treated C6 cells
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81219-2-RR targets Phospho-SHP2 (Tyr580) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 11
别名 P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)
计算分子量 597 aa, 68 kDa
观测分子量72 kDa
GenBank蛋白编号BC008692
基因名称 PTPN11
Gene ID (NCBI) 5781
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ06124
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)

实验方案

Product Specific Protocols
WB protocol for Phospho-SHP2 (Tyr580) antibody 81219-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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