• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 84563-1-RR

    WB analysis using 84563-1-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84563-1-RR (Phospho-SH3PXD2B (Ser291) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • IF Staining of HeLa using 84563-1-RR

    IF Staining of HeLa using 84563-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-SH3PXD2B (Ser291) antibody (84563-1-RR, Clone: 241064C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-SH3PXD2B (Ser291) Recombinant monoclonal antibody

Phospho-SH3PXD2B (Ser291) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84563-1-RR

产品说明书

CloneNo. 241064C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Phospho-SH3PXD2B, Phospho-SH3PXD2B Ser291, P-SH3PXD2B, P-SH3PXD2B (Ser291), SH3PXD2B (Ser291)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84563-1-RR targets Phospho-SH3PXD2B (Ser291) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SH3 and PX domains 2B
别名 Phospho-SH3PXD2B, Phospho-SH3PXD2B Ser291, P-SH3PXD2B, P-SH3PXD2B (Ser291), SH3PXD2B (Ser291)
计算分子量911aa, 102 kDa
观测分子量120 kDa
GenBank蛋白编号NM_001017995
基因名称 SH3PXD2B
Gene ID (NCBI) 285590
RRIDAB_3672071
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDA1X283
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3 and PX domains 2B (SH3PXD2B) is also known as tyrosine kinase substrate with four SH3 domains (Tks4). It consists of the N-terminal phox homology domain, four Sr homology 3 domains, several proline-rich regions, and Src phosphorylation sites. Previous studies have shown that SH3PXD2B is an essential invadopodium scaffold protein and can stimulate actin polymerization, recruit membrane type-1 matrix metalloproteinase (MT1-MMP) to intracellular membranes, and increase Nox1-dependent reactive oxygen species (ROS) production. (PMID: 33906640)

实验方案

Product Specific Protocols
WB protocol for Phospho-SH3PXD2B (Ser291) antibody 84563-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...