Phospho-RPS6KA1 (Ser380) Recombinant antibody
Phospho-RPS6KA1 (Ser380) Recombinant Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
7F23
验证数据展示
经过测试的应用
Positive WB detected in | PDGF treated NIH/3T3 cells, HeLa cells, Jurkat cells, Calyculin A treated HeLa cells, Calyculin A treated Jurkat cells |
Positive IHC detected in | human malignant melanoma tissue, Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | TPA treated Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80108-1-RR targets Phospho-RPS6KA1 (Ser380) in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications | FC, IHC, WB, ELISA |
Tested Reactivity | Human, Mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
Synonyms | HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Ser380), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1 |
Calculated Molecular Weight | 735 aa, 83 kDa |
Observed Molecular Weight | 83-90 kDa |
GenBank Accession Number | BC014966 |
Gene Symbol | RPS6KA1 |
Gene ID (NCBI) | 6195 |
RRID | AB_2918865 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15418 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-RPS6KA1 (Ser380) antibody 80108-1-RR | Download protocol |
IHC protocol for Phospho-RPS6KA1 (Ser380) antibody 80108-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |