验证数据展示
经过测试的应用
| Positive WB detected in | Insulin treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28928-1-AP targets Phospho-PRKD1 (Ser916) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase D1 |
| 别名 | Phospho-PRKD1 (Ser916), PKC MU, PKCM, PKD, PKD1, PRKCM, PRKD1, Protein kinase C mu type, Protein kinase D, protein kinase D1 |
| 计算分子量 | 102 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | NM_001330069 |
| 基因名称 | PRKD1 |
| Gene ID (NCBI) | 5587 |
| RRID | AB_2918215 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15139 |
| 储存缓冲液 | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PRKD1 (Ser916) antibody 28928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Chem Toxicol G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes. | ||
Biochem Biophys Res Commun Protein kinase D1 promotes the survival of random-pattern skin flaps in rats |