验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29874-1-AP targets Phospho-PPP1CA (Thr320) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, catalytic subunit, alpha isoform |
| 别名 | Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA |
| 计算分子量 | 330 aa, 38 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC001888 |
| 基因名称 | PPP1CA |
| Gene ID (NCBI) | 5499 |
| RRID | AB_2935422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62136 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PPP1CA (Thr320) antibody 29874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
