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验证数据展示

  • WB analysis using 29107-1-AP

    WB analysis using 29107-1-AP

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

Phospho-PKN1 (Thr774) Polyclonal antibody

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, ELISA
Cat No. 29107-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPDGF treated NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase N1
别名 DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha
计算分子量 942 aa, 104 kDa
观测分子量 130 kDa
GenBank蛋白编号BC040061
基因名称 PKN1
Gene ID (NCBI) 5585
RRIDAB_2918235
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)

实验方案

Product Specific Protocols
WB protocol for Phospho-PKN1 (Thr774) antibody 29107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols