验证数据展示
经过测试的应用
| Positive WB detected in | PDGF treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase N1 |
| 别名 | DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha |
| 计算分子量 | 942 aa, 104 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC040061 |
| 基因名称 | PKN1 |
| Gene ID (NCBI) | 5585 |
| RRID | AB_2918235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16512 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PKN1 (Thr774) antibody 29107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
