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验证数据展示

  • WB analysis using 29107-1-AP

    WB analysis using 29107-1-AP

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

  • IF Staining of NIH/3T3 using 29107-1-AP

    IF Staining of NIH/3T3 using 29107-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PDGF treated NIH/3T3 cells using Phospho-PKN1 (Thr774) antibody (29107-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-PKN1 (Thr774) Polyclonal antibody

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29107-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

p PKN1, p PKN1 (Thr774), p PKN1 Thr774, Phospho PKN1, Phospho PKN1 (Thr774)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPDGF treated NIH/3T3 cells
Positive IF/ICC detected inPDGF treated NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29107-1-AP targets Phospho-PKN1 (Thr774) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase N1
别名 p PKN1, p PKN1 (Thr774), p PKN1 Thr774, Phospho PKN1, Phospho PKN1 (Thr774)
计算分子量 942 aa, 104 kDa
观测分子量 130 kDa
GenBank蛋白编号BC040061
基因名称 PKN1
Gene ID (NCBI) 5585
RRIDAB_2918235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)

实验方案

Product Specific Protocols
IF protocol for Phospho-PKN1 (Thr774) antibody 29107-1-APDownload protocol
WB protocol for Phospho-PKN1 (Thr774) antibody 29107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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