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Phospho-PKN1 (Thr774) Polyclonal antibody

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29107-1-AP

Print datasheet

Synonyms

DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha

验证数据展示

  • WB analysis using 29107-1-AP

    WB analysis using 29107-1-AP

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

经过测试的应用

Positive WB detected inPDGF treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase N1
Synonyms DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha
Calculated Molecular Weight 942 aa, 104 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC040061
Gene Symbol PKN1
Gene ID (NCBI) 5585
RRIDAB_2918235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)

实验方案

Product Specific Protocols
WB protocol for Phospho-PKN1 (Thr774) antibody 29107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols