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验证数据展示

  • WB analysis using 87213-1-RR (same clone as 87213-1-PBS)

    WB analysis using 87213-1-RR (same clone as 87213-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.

  • WB analysis using 87213-1-RR (same clone as 87213-1-PBS)

    WB analysis using 87213-1-RR (same clone as 87213-1-PBS)

    Non-treated A549 cells, phosphatase inhibitor treated A549 cells and λ phosphatase treated A549 cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 87213-1-RR (same clone as 87213-1-PBS)

    FC experiment of A549 using 87213-1-RR (same clone as 87213-1-PBS)

    1X10^6 A549 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR, Clone:252421F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.

Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody, PBS Only

Phospho-PKC Delta (Ser645) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 87213-1-PBS

产品说明书

CloneNo. 252421F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

Phospho-PKC Delta, Phospho-PKC Delta (Ser643), PKC Delta (Ser643), PKC Delta (Ser645), p-PKC Delta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

产品信息

87213-1-PBS targets Phospho-PKC Delta (Ser645) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase C, delta
别名 Phospho-PKC Delta, Phospho-PKC Delta (Ser643), PKC Delta (Ser643), PKC Delta (Ser645), p-PKC Delta
计算分子量 78 kDa
观测分子量78 kDa
GenBank蛋白编号NM_006254
基因名称 PKC Delta
Gene ID (NCBI) 5580
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ05655
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. Phospho-PKC Delta (Ser645) refers to the phosphorylated form of Protein Kinase C Delta (PKCδ) at serine residue 645. This phosphorylation site is located in the C-terminal tail of PKCδ, specifically in the turn motif region, and serves as a critical "priming" phosphorylation that regulates enzyme stability and catalytic activity. (PMID: 28784722) This antibody is equivalent to Ser643 in mice.

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