验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HeLa cells |
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG).
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87365-3-RR targets Phospho-PKA C (Thr198) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, catalytic, alpha |
| 别名 | Phospho-PKA C (Thr197), Phos-PKA C, PKA C-beta, PKA C-gamma, P-PKA C |
| 计算分子量 | 41 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | NM_002730 |
| 基因名称 | PRKACA |
| Gene ID (NCBI) | 5566 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17612 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ).(PMID: 36431013, PMID: 39592424)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
