验证数据展示
经过测试的应用
| Positive WB detected in | λ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 27 publications below |
| IHC | See 1 publications below |
产品信息
29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, zebrafish, bovine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2-alpha kinase 3 |
| 别名 | EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3 |
| 计算分子量 | 1116 aa, 125 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC126354 |
| 基因名称 | PERK |
| Gene ID (NCBI) | 9451 |
| RRID | AB_2935416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 29546-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mar Drugs Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma | ||
World J Gastroenterol Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress | ||
Prostaglandins Other Lipid Mediat Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress | ||
Pharmaceuticals (Basel) Maltol Improves Peripheral Nerve Function by Inhibiting Schwann Cell Apoptosis via the PERK/eIF2α/CHOP Pathway and MME Upregulation in Diabetic Peripheral Neuropathy | ||
Ecotoxicol Environ Saf Endoplasmic reticulum stress promotes oxidative stress, inflammation, and apoptosis: A novel mechanism of citrinin-induced renal injury and dysfunction | ||
Am J Physiol Heart Circ Physiol One day of environment-induced heat stress damages the murine myocardium |