Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA
Cat No. 29546-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (4)

应用

WB, ELISA and More (1)

EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells, Thapsigargin treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse, rat, zebrafish, bovine
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3
计算分子量 1116 aa, 125 kDa
观测分子量140 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
RRIDAB_2935416
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

实验方案

Product Specific Protocols
WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 29546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mar Drugs

Ascomylactam C Induces an Immunogenic Cell Death Signature via Mitochondria-Associated ER Stress in Lung Cancer and Melanoma

Authors - Yun Huang
humanWB

World J Gastroenterol

Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress

Authors - Xiao-Xue Hou
mouse,ratWB

Prostaglandins Other Lipid Mediat

Dahuang Huanglian Xiexin Decoction ameliorates obesity via modulating adipocyte differentiation and lipid degradation through inhibiting endoplasmic reticulum stress

Authors - Long Hao
ratWB

Pharmaceuticals (Basel)

Maltol Improves Peripheral Nerve Function by Inhibiting Schwann Cell Apoptosis via the PERK/eIF2α/CHOP Pathway and MME Upregulation in Diabetic Peripheral Neuropathy

Authors - Jiawei Li
mouseWB

Ecotoxicol Environ Saf

Endoplasmic reticulum stress promotes oxidative stress, inflammation, and apoptosis: A novel mechanism of citrinin-induced renal injury and dysfunction

Authors - Yongkang Wang
mouseWB

Am J Physiol Heart Circ Physiol

One day of environment-induced heat stress damages the murine myocardium

Authors - Melissa Roths
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