验证数据展示
经过测试的应用
| Positive WB detected in | PDGF treated PC-3 cells, PDGF treated NIH/3T3 cells |
| Positive IF/ICC detected in | PDGF treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29241-1-AP targets Phospho-PDPK1 (Ser241) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3-phosphoinositide dependent protein kinase-1 |
| 别名 | p PDPK1, p PDPK1 (Ser241), p PDPK1 Ser241, PDPK1 (Ser241), Phospho PDPK1 |
| 计算分子量 | 556 aa, 63 kDa |
| 观测分子量 | 58-68 kDa |
| GenBank蛋白编号 | BC012103 |
| 基因名称 | PDPK1 |
| Gene ID (NCBI) | 5170 |
| RRID | AB_2918256 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15530 |
| 储存缓冲液 | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-PDPK1 (Ser241) antibody 29241-1-AP | Download protocol |
| WB protocol for Phospho-PDPK1 (Ser241) antibody 29241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Penfluridol directly targets PDPK1 to suppress AKT1 phosphorylation and stabilize CTR1, inducing cuproptosis in colorectal cancer. | ||
Cell Rep A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism | ||
Adv Sci (Weinh) Corynoxine Inhibits Tumor Growth via Targeting NQO1 and Modulating the PTPA-NQO1/PP2A Switch to Activate PP2A. |
