验证数据展示
经过测试的应用
| Positive WB detected in | MG132 treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29503-1-AP targets Phospho-P62/SQSTM1 (Ser349) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sequestosome 1 |
| 别名 | p p62 (Ser349), p p62 Ser349, p P62,SQSTM1, p P62,SQSTM1 (Ser349), p P62,SQSTM1 Ser349 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC017222 |
| 基因名称 | P62/SQSTM1 |
| Gene ID (NCBI) | 8878 |
| RRID | AB_2923592 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13501 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-P62/SQSTM1 (Ser349) antibody 29503-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Butyrate protects against Klebsiella pneumoniae-induced oxidative stress in alveolar macrophages via p62-Keap1-Nrf2 pathway.
| ||
Adv Sci (Weinh) Modulating Macrophage-Nucleus Pulposus Cell Crosstalk via Sequential Drug Delivery Attenuates Disc Degeneration | ||
Adv Sci (Weinh) Targeting GPX4 to Induce Ferroptosis Overcomes Chemoresistance Mediated by the PAX8-AS1/GPX4 Axis in Intrahepatic Cholangiocarcinoma | ||
Cell Death Differ SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
Food Res Int WIPButyrate produced by the Lycium ruthenicum polysaccharide alleviated sleep deprivation-induced chronic fatigue syndrome in mice through promoting microglial autophagy. |
