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验证数据展示

  • WB analysis using 80077-9-RR

    WB analysis using 80077-9-RR

    Non-treated HT-29 cells and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • WB analysis of A431 using 80077-9-RR

    WB analysis of A431 using 80077-9-RR

    Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • IF Staining of HT-29 using 80077-9-RR

    IF Staining of HT-29 using 80077-9-RR

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser46) antibody (80077-9-RR, Clone: 250307A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-P53 (Ser46) Recombinant monoclonal antibody

Phospho-P53 (Ser46) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80077-9-RR

产品说明书

CloneNo. 250307A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inetoposide treated HT-29 cells, nocodazole treated A431 cells
Positive IF/ICC detected inetoposide treated HT-29 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80077-9-RR targets Phospho-P53 (Ser46) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53
别名 Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site.

实验方案

Product Specific Protocols
IF protocol for Phospho-P53 (Ser46) antibody 80077-9-RRDownload protocol
WB protocol for Phospho-P53 (Ser46) antibody 80077-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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