验证数据展示
经过测试的应用
| Positive WB detected in | etoposide treated T-47D cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87312-1-RR targets Phospho-P53 (Ser33) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor protein p53 |
| 别名 | Phospho P53, Phospho P53 (Ser33), Phospho-P53, Phospho-P53-Ser33, p-p53 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | NM_000546 |
| 基因名称 | P53 |
| Gene ID (NCBI) | 7157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04637 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. Phosphorylation of p53 at specific amino acid residues plays a crucial role in mediating its various functions, including its role as a transcription factor that regulates the expression of genes involved in the cellular response to stress and DNA damage. Upon severe DNA damage, p53 is phosphorylated at specific amino acid residues, becomes stabilized, and activated to induce apoptosis-related genes. To date, 10 kinases have been identified to phosphorylate p53 for apoptosis at specific serine/threonine residues in the N- and C-terminus domains. (PMID: 30191640)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-P53 (Ser33) antibody 87312-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
