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验证数据展示

  • WB analysis using 28851-1-AP

    WB analysis using 28851-1-AP

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28851-1-AP (Phospho-NF2 (Ser518) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-Neurofibromin 2 (Ser518) Polyclonal antibody

Phospho-Neurofibromin 2 (Ser518) Polyclonal Antibody for WB, ELISA
Cat No. 28851-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Phospho-NF2 (Ser518), NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin 2, Neurofibromin-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28851-1-AP targets Phospho-Neurofibromin 2 (Ser518) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurofibromin 2 (merlin)
别名 Phospho-NF2 (Ser518), NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin 2, Neurofibromin-2
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007279
基因名称 NF2
Gene ID (NCBI) 4771
RRIDAB_2935410
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35240
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. 28851-1-AP antibody is specific to phosphorylation of NF2 at serine 518. (PMID: 11782491, 14724586, 18361411, 18835652, 32115406)

实验方案

Product Specific Protocols
WB protocol for Phospho-Neurofibromin 2 (Ser518) antibody 28851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols