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验证数据展示

  • WB analysis of C2C12 using 85141-5-RR (same clone as 85141-5-PBS)

    WB analysis of C2C12 using 85141-5-RR (same clone as 85141-5-PBS)

    Non-treated C2C12 cells and Insulin treated C2C12 cells were subjected to SDS PAGE followed by western blot with 85141-5-RR (Phospho-NDRG1 (Thr346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85141-5-PBS in a different storage buffer formulation.

Phospho-NDRG1 (Thr346) Recombinant antibody, PBS Only

Phospho-NDRG1 (Thr346) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85141-5-PBS

产品说明书

CloneNo. 250220C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

NDRG1 (Thr346), p NDRG1, p NDRG1 (Thr346), Phospho NDRG1, Phospho NDRG1 (Thr346)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85141-5-PBS targets Phospho-NDRG1 (Thr346) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-myc downstream regulated 1
别名 NDRG1 (Thr346), p NDRG1, p NDRG1 (Thr346), Phospho NDRG1, Phospho NDRG1 (Thr346)
计算分子量 43 kDa
观测分子量46 kDa, 48 kDa
GenBank蛋白编号BC006260
基因名称 NDRG1
Gene ID (NCBI) 10397
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92597
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-Myc Downstream Regulated 1 (NDRG1) is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. The phosphorylation of NDRG1 (on Thr346) showed the greatest statistically significant increase in clear cell renal cell carcinoma (ccRCC) compared with noncancerous renal tissue. Further study showed the phosphorylation of NDRG1 (Thr346) in ccRCC cells was maintained by RICTOR and mTOR in complex 2. (PMID: 37298315)