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验证数据展示

  • WB analysis of HEK-293 using 85804-1-RR (same clone as 85804-1-PBS)

    WB analysis of HEK-293 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 85804-1-RR (same clone as 85804-1-PBS)

    WB analysis of NIH/3T3 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 85804-1-RR (same clone as 85804-1-PBS)

    WB analysis of HSC-T6 using 85804-1-RR (same clone as 85804-1-PBS)

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant antibody, PBS Only

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85804-1-PBS

产品说明书

CloneNo. 250098F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

MYL12B, MYL9, P MYL12A, P MYL12B, P Myosin Light Chain 2 (Thr18/Ser19)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYL12A Ready-To-Use IHC Kit. MYL12A primary antibody included.

产品信息

85804-1-PBS targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, light chain 12A, regulatory, non-sarcomeric
别名 MYL12B, MYL9, P MYL12A, P MYL12B, P Myosin Light Chain 2 (Thr18/Ser19)
计算分子量 171 aa, 20 kDa
观测分子量 19-20 kDa
GenBank蛋白编号BC016372
基因名称 MYL12A
Gene ID (NCBI) 10627
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19105
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.