验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells, λ phosphatase treated PC-12 cells |
Positive IF/ICC detected in | λ phosphatase treated U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, rat |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | MOB1, Mps One Binder kinase activator-like 1B (yeast) |
别名 | MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B |
计算分子量 | 216 aa, 25 kDa |
观测分子量 | 25 kDa |
GenBank蛋白编号 | BC003398 |
基因名称 | MOBKL1B |
Gene ID (NCBI) | 55233 |
RRID | AB_2923589 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H8S9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-AP | Download protocol |
IF protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |