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验证数据展示

  • WB analysis using 29027-1-AP

    WB analysis using 29027-1-AP

    Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of U2OS using 29027-1-AP

    IF Staining of U2OS using 29027-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated U2OS cells using Phospho-MOBKL1B (Thr12) antibody (29027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-MOBKL1B (Thr12) Polyclonal antibody

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29027-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat and More (1)

应用

WB, IF/ICC, ELISA

别名

MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells, λ phosphatase treated PC-12 cells
Positive IF/ICC detected inλ phosphatase treated U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MOB1, Mps One Binder kinase activator-like 1B (yeast)
别名 MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B
计算分子量 216 aa, 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC003398
基因名称 MOBKL1B
Gene ID (NCBI) 55233
RRIDAB_2923589
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8S9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).

实验方案

Product Specific Protocols
IF protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-APDownload protocol
WB protocol for Phospho-MOBKL1B (Thr12) antibody 29027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Cell Physiol

m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma

Authors - Jiamei Wu
View Article
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