验证数据展示
经过测试的应用
| Positive WB detected in | λ phosphatase treated HeLa cells, HEK-293 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68050-1-Ig targets Phospho-MKK7 (Ser271/Thr275) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 7 |
| 别名 | JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47-52 kDa |
| GenBank蛋白编号 | BC038295 |
| 基因名称 | MKK7 |
| Gene ID (NCBI) | 5609 |
| RRID | AB_2918792 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O14733 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MKK7 (Ser271/Thr275) antibody 68050-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
