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验证数据展示

  • WB analysis of NIH/3T3 using 84556-1-RR

    WB analysis of NIH/3T3 using 84556-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84556-1-RR (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-MEK2 (Thr394) Recombinant antibody

Phospho-MEK2 (Thr394) Recombinant Antibody for WB, ELISA
Cat No. 84556-1-RR

产品说明书

CloneNo. 241669E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MEK2 (Thr394), Phospho MEK2 (Thr394), Phospho-MEK2, p-MEK2, p-MEK2 (Thr394)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84556-1-RR targets Phospho-MEK2 (Thr394) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 2
别名 MEK2 (Thr394), Phospho MEK2 (Thr394), Phospho-MEK2, p-MEK2, p-MEK2 (Thr394)
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC018645
基因名称 MEK2
Gene ID (NCBI) 5605
RRIDAB_3672065
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36507
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK2 (Thr394) antibody 84556-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols