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验证数据展示

  • WB analysis using 28935-1-AP

    WB analysis using 28935-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28935-1-AP (Phospho-MEK1 (Thr386) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28935-1-AP

    IF Staining of HeLa using 28935-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-MEK1 (Thr386) antibody (28935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-MEK1 (Thr386) Polyclonal antibody

Phospho-MEK1 (Thr386) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28935-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28935-1-AP targets Phospho-MEK1 (Thr386) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 1
别名 MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1
计算分子量 43 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC139729
基因名称 MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
RRIDAB_2918218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02750
储存缓冲液 PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. MEK1 may form a heterodimer with MEK2.(PMID:21144847,19219045).

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK1 (Thr386) antibody 28935-1-APDownload protocol
IF protocol for Phospho-MEK1 (Thr386) antibody 28935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols