验证数据展示
经过测试的应用
| Positive WB detected in | PMA treated HeLa cells, starve overnight treated and PMA treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MAPKAPK2 Ready-To-Use IHC Kit. MAPKAPK2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87209-1-RR targets Phospho-MAPKAPK2 (Thr222) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase-activated protein kinase 2 |
| 别名 | MAPKAP Kinase 2 (phospho Thr222), Phospho MAPKAPK2, Phospho MAPKAPK2 (Thr222), phospho-MAPKAP Kinase 2 (Thr222), Phospho-MAPKAPK2 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | NM_032960 |
| 基因名称 | MAPKAPK2 |
| Gene ID (NCBI) | 9261 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49137 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. Phospho-MAPKAPK2 (Thr222) is a phosphorylated form of MAPKAPK2 (MAPK-Activated Protein Kinase 2), which is also known as MK2. MAPKAPK-2 (MK2) is a direct target of p38 MAPK and is activated through phosphorylation at multiple residues, including Thr222, Ser272, and Thr334. (PMID: 20596525)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MAPKAPK2 (Thr222) antibody 87209-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
