验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
28844-1-AP targets Phospho-MAP3K1 (Thr1400) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase kinase 1 |
| 别名 | MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MEK kinase 1, MEKK, MEKK 1, MEKK1, Phospho-MAP3K1 (Thr1400) |
| 计算分子量 | 164 kDa |
| 观测分子量 | 164 kDa |
| GenBank蛋白编号 | NM_005921 |
| 基因名称 | MAP3K1 |
| Gene ID (NCBI) | 4214 |
| RRID | AB_2918209 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13233 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. MAP3K1 is activated by autophosphorylation on Thr-1400 and Thr-1412(Uniprot).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MAP3K1 (Thr1400) antibody 28844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Resveratrol inhibits autophagy against myocardial ischemia-reperfusion injury through the DJ-1/MEKK1/JNK pathway | ||
Cell Death Dis CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer |