验证数据展示
经过测试的应用
| Positive WB detected in | PDGF treated PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
29250-1-AP targets Phospho-MAP2K4 (Ser257/Thr261) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 4 |
| 别名 | JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, MKK4, Phospho-MAP2K4, Phospho-MAP2K4 (Ser257/Thr261), P-MAP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1 |
| 计算分子量 | 399 aa, 44 kDa |
| 观测分子量 | 44-50 kDa |
| GenBank蛋白编号 | BC036032 |
| 基因名称 | MAP2K4 |
| Gene ID (NCBI) | 6416 |
| RRID | AB_2918258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P45985 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MAP2K4 (Ser257/Thr261) antibody 29250-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity | ||
J Inorg Biochem Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis | ||
Bioorg Med Chem Synthesis and biological evaluation of lappaconitine analogues as potential anti-neuroinflammatory agents by side chain modification and scaffold hopping strategy |