验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87289-1-RR targets Phospho-LAT (Tyr220) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | linker for activation of T cells |
| 别名 | LAT (Tyr220), Phospho LAT (Tyr220), Phospho-LAT, p-LAT (Tyr220), p-LAT (Y220) |
| 计算分子量 | 262 aa, 28 kDa |
| 观测分子量 | 36 kDa, 38 kDa |
| GenBank蛋白编号 | BC011563 |
| 基因名称 | LAT |
| Gene ID (NCBI) | 27040 |
| RRID | AB_3745449 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43561 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. Phospho-LAT (Tyr220) is a phosphorylated form of the Linker for Activation of T cells (LAT), a protein that plays a pivotal role in T cell receptor (TCR) signaling. LAT is involved in the assembly of the signaling complex that leads to T cell activation. Upon TCR engagement, LAT becomes phosphorylated at multiple tyrosine residues, including Tyr220. (PMID: 38746248)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-LAT (Tyr220) antibody 87289-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
