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验证数据展示

  • ChIP experiment of HeLa using 82828-15-RR

    ChIP experiment of HeLa using 82828-15-RR

    Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser28) (82828-15-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis of HeLa using 82828-15-RR

    WB analysis of HeLa using 82828-15-RR

    Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-15-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody

Phospho-Histone H3 (Ser28) Uni-rAb® Recombinant Antibody for WB, ELISA, ChIP-qPCR
Cat No. 82828-15-RR

产品说明书

CloneNo. 250369A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA, ChIP-qPCR

别名

H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected innocodazole treated HeLa cells
Positive ChIP-qPCR detected inNocodazole-HeLa (100 ng/ml, 16 h) HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82828-15-RR targets Phospho-Histone H3 (Ser28) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histone H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and β-globin genes (PMID: 17913747).

实验方案

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Ser28) antibody 82828-15-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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