验证数据展示
经过测试的应用
| Positive WB detected in | nocodazole treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86873-1-RR targets Phospho-Histone H2B (Ser32) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 2, H2be |
| 别名 | H2B, H2B Phos Ser32, H2B Phospho S32, H2B Phospho Ser32, H2B Phospho Serine9 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC005827 |
| 基因名称 | Histone H2B |
| Gene ID (NCBI) | 8349 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16778 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
During mitosis, Aurora-B directly binds and phosphorylates Histone H2B at Ser32. (PMID: 29563611)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Histone H2B (Ser32) antibody 86873-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
