验证数据展示
经过测试的应用
| Positive WB detected in | dexamethasone treated PC-12 cells |
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87413-2-RR targets Phospho-Glucocorticoid receptor (Ser211) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
| 别名 | Glucocorticoid receptor, NR3C1, GCCR, GCR, GR |
| 计算分子量 | 86 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | BC015610 |
| 基因名称 | Glucocorticoid receptor |
| Gene ID (NCBI) | 2908 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04150 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Ser211 is located within the hinge region of the GR, which connects the DNA-binding domain and the ligand-binding domain. Phosphorylation at Ser211 is crucial for the receptor's transcriptional activity and nuclear translocation. (PMID: 40362450)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Glucocorticoid receptor (Ser211) antibody 87413-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
