验证数据展示
经过测试的应用
| Positive WB detected in | λ phosphatase treated PC-3 cells |
| Positive IF/ICC detected in | λ phosphatase treated PC-3 cells |
This antibody can recognize the Ser641 site of both GYS1 and GYS2.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28855-1-AP targets Phospho-GYS (Ser641) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycogen synthase 1 (muscle) |
| 别名 | GYS2, p GYS1 , p GYS1 (Ser641), p GYS1 Ser641, p-GYS1 |
| 计算分子量 | 84 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC007688 |
| 基因名称 | GYS1 |
| Gene ID (NCBI) | 2997 |
| RRID | AB_2923584 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P13807 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-GYS (Ser641) antibody 28855-1-AP | Download protocol |
| IF protocol for Phospho-GYS (Ser641) antibody 28855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |