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Phospho-EPHA2 (Tyr588) Polyclonal antibody

Phospho-EPHA2 (Tyr588) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30263-1-AP

Print datasheet

Synonyms

ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase

验证数据展示

  • WB analysis using 30263-1-AP

    WB analysis using 30263-1-AP

    Non-treated and MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 30263-1-AP (Phospho-EPHA2 (Tyr588) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

经过测试的应用

Positive WB detected inMG132 treated HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30263-1-AP targets Phospho-EPHA2 (Tyr588) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EPH receptor A2
Synonyms ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase
Calculated Molecular Weight 976 aa, 108 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC037166
Gene Symbol EPHA2
Gene ID (NCBI) 1969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29317
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ephrin type-A receptor 2 (EPHA2), belongs to the receptor tyrosine kinases (RTKs) family, that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (PMID: 11114742). The "reverse signaling" function, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allows interactions with other proteins that may activate signaling pathways in the ligand-expressing cells. The detected molecular weight is around 110 kDa.

实验方案

Product Specific Protocols
WB protocol for Phospho-EPHA2 (Tyr588) antibody 30263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols