验证数据展示
经过测试的应用
| Positive WB detected in | EGF treated HeLa cells |
This antibody, also known as EGFR Tyr1045, is the site where the signal peptide is removed.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30277-1-AP targets Phospho-EGFR (Tyr1069) in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| 别名 | EGFR (Tyr1069), p EGFR , p EGFR (Tyr1069), p EGFR Tyr1069, p-EGFR |
| 计算分子量 | 1210 aa, 134 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC094761 |
| 基因名称 | EGFR |
| Gene ID (NCBI) | 1956 |
| RRID | AB_3085349 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-EGFR (Tyr1069) antibody 30277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Engineered bacterial outer membrane vesicles enhanced tumor immunotherapy through remodeling tumor stroma and targeted delivery of CD73 siRNA. | ||
Pharmacol Res ACSS2 drives Lenvatinib resistance in hepatocellular carcinoma through palmitoylation. | ||
Cell Death Dis PLSCR1 drives chemoresistance in TNBC via METTL3/IGF2BP3-mediated mRNA stabilization and EGFR-MAPK pathway activation. | ||
Antioxidants (Basel) Danthron Attenuates Intestinal Inflammation by Modulating Oxidative Stress via the EGFR-PI3K-AKT and Nrf2-HO-1 Pathways. | ||
J Autoimmun Mechanistic studies on the regulation of systemic sclerosis progression by imbalance in angiogenic T cell subsets. | ||
Ecotoxicol Environ Saf DEHP exposure and cardiac fibrosis risk: An integrated network toxicology and molecular experimental investigation. |
